A sperm penetration assay SPA is a lab test used to measure sperm ability to undergo capacitation— a set of physiological changes that sperm must undergo to be able to penetrate the egg and fertilize it. The test calculates the percentage of pretreated hamster eggs that are penetrated by human sperm. SPA is not frequently performed and is unlikely to significantly alter treatment recommendations. Sperm penetration assay is also known as a hamster test, hamster penetration test, and zona-free hamster oocyte penetration assay. In order to predict the capacity of sperm to fertilize an egg, a sample of sperm is mixed with prepared hamster eggs in a laboratory. The hamster eggs are chemically treated so that human sperm could penetrate them.
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Vitamin D and Human Sperm DNA Fragmentation
Sperm MTT Viability Assay: A New Method for Evaluation of Human Sperm Viability | SpringerLink
To determine whether acrosome function scoring—including acrosomal enzyme AE levels and acrosome reaction AR results—can predict fertilization rate in vitro. Additionally, a meta-analysis was done for prospective cohort or case-control studies; the following summary measures were reported to expand upon the findings: pooled spearman correlation coefficient Rs , standardized mean difference SMD , sensitivity SEN , specificity SPE , positive likelihood ratio PLR , negative likelihood ratio NLR , diagnostic score DS , diagnostic odds ratio DOR , and area under the summary receiver operating characteristic curve AUC. On meta-analysis, a total of 44 unique articles were selected, but given the multiple techniques described there was a total of 67 total datasets extracted from these 44 articles, comprising infertile couples undergoing IVF therapy. Lower AE levels determined by fluorometry or substrate assay were used for predicting lower FR with low sensitivity and high specificity; the spectrophotometry assay had an uncertain predictive value.
Hamster zona-free ovum test
Plate A is a light photomicrograph of sperm smear and green sperm heads in plate B show damage to sperm DNA under fluoroscent microscopy. Nuclear transfer in Mouse Oocytes: A - lancing of zona pellucida, B and C - removal of germinal vesicle, D - an isolated germinal vesicle. This photo shows a human blastocyst - an embryo days old.